科学会議

The development of approaches for rational design and rapid reliable analysis of the properties of new nucleic acid derivatives is an urgent problem in chemistry and physical chemistry. We have developed approaches for the experimental characterization of the physicochemical properties of short oligomers of newly synthetized nucleic acid derivatives, and have tested computer simulation approaches for the prediction of their complexes structure and hybridization properties.

The Ngari virus (NRIV) is a mosquito-borne virus of the genus Orthobunyavirus. This virus is pathogenic for humans and associated with hemorrhagic fever. We are detected of the Ngari virus in ixodid ticks collected from cattle in Guinea. The Tintin/Kankan/Guinea/2018 and Sabadou/Kankan/Guinea/2018 strains of the Ngari virus were isolated with using Vero cells. The virus infectious titer was 107 TCID50/ml. The sequences of complete genome of the NRIV were determined. The length of the NRIV genomes are 12 269 bp. The level of the nucleotide sequence identity with the closest Adrar strain of NRIV was 96% (S-segment) and 98% (M, L-segments), respectively. Moreover, these strains were clustered into a separate genetic group on the phylogenetic tree.

T-cell malignancies form a small part of hematological diseases, however, they are characterized by high mortality rate and treated poorly. The immunotherapy with T- or NK-cells with chimeric antigen receptors (CAR) is a prospective treatment method for T-cell lymphomas. CAR is a chimeric molecule, its extracellular part recognizes tumor antigens, and intracellular one triggers a cytotoxic reaction. One of the most attractive targets for the elimination of malignant T-cells is the surface protein CD5, but it is also expressed on normal T-lymphocyte cells, which makes it hard to create a CAR T cell preparation. To overcome this limitation, we propose to use CD5-specific CAR NK cells.

DNA-based vaccines were engineered containing genes of SARS-CoV-2 S protein and its receptor-binding domain (RBD). Following immunization of mice antigen-specific antibodies with high neutralization activity and T-cell response were measured.